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Catalog Number | orb1272746 |
---|---|
Category | Antibodies |
Description | IL-22 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Mouse |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-22. Murine IL-22 specific antibody was purified by affinity chromatography employing immobilized mIL-22 matrix. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
Target | Il22 |
UniProt ID | Q9JJY9 |
NCBI | Q9JJY9 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | IL-22, Iltif, IL-22a, ILTIFa, Il22a, Iltifa, Inter Read more... |
Note | For research use only |
Application notes | ELISA:Direct:To detect mIL-22 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.SandwichTo detect mIL-22 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-22 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22. Western Blot:To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Expiration Date | 12 months from date of receipt. |
To detect mIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-22 (orb1272745) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Canine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |