IL-21 Antibody

• Catalog Number: orb1272683
• Category: Antibodies
• Description: IL-21 Antibody
• Target: Il21
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21). Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix.
• UniProt ID: Q9ES17
• Tested applications: ELISA, WB
• Application notes: ELISA:Indirect:To detect mIL-21 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.SandwichTo detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-21 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21. Western Blot:To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-21IL-21
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: Q9ES17

To detect mIL-21 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-21 (orb1272682) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.