IL-20R alpha Rabbit Polyclonal Antibody

SKU: orb1291527

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Description

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant human IL-20R alpha protein (30-250/553aa)
Target	IL20RA
Molecular Weight	59 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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IL-20Rα; Interleukin-20 receptor alpha chain precursor; IL-20RA; IL20 RA; IL-20 RA; E230031K19; FLJ40993; IL-20R1; IL20RA; ZCYTOR7; Interleukin-20 receptor subunit alpha; IL-20 receptor subunit alpha; IL-20R-alpha; IL-20RA; Cytokine receptor family 2 member 8; CRF2-8; Cytokine receptor class-II member 8; I20RA_HUMAN.

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Measured by its binding ability in a indirect ELISA. Immobilized Human IL-20R alpha protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human IL-20R alpha Antibody, the EC50 is 2249 ng/ml.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Rat Breast tissue lysates, Lane 4: Human SH-SY5Y cell lysates, Lane 5: Human A431 cell lysates, Primary: Anti-IL-20R alpha (orb1291527) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kDa, Observed band size: 59 kDa.

Sample: Lane 1: Recombinant human IL-20R alpha protein, Primary: Anti-IL-20R alpha (orb1291527) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kDa, Observed band size: 28 kDa.

Quick Database Links

Gene Symbol

IL20RA

UniProt

Q9UHF4

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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