IL-17E antibody

• Catalog Number: orb345113
• Category: Antibodies
• Description: IL-17E antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 11K8B1
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
• Concentration: 1.0mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, FC: 1µg/mL, WB: 0.5µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-IL-17E was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q9H293
• NCBI: NP_073626.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse Anti-IL-17E Antibody, mouse anti-IL-25 antib
• Note: For research use only
• Application notes: IL 17E antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of full length recombinant protein using IL-17E antibody