IL-17E antibody (Biotin)

• Catalog Number: orb345120
• Category: Antibodies
• Description: IL-17E antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 11K8B1
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, FC: 1µg/mL, WB: 0.5µg/mL
• Form/Appearance: Lyophilized
• Purity: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined.
• Conjugation: Biotin
• UniProt ID: Q9H293
• NCBI: NP_073626.1
• Storage: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse Anti-IL-17E Biotin Conjugated Antibody, mous
• Note: For research use only
• Application notes: This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of PC3 cells using IL-17E antibody (Biotin)

Western blot analysis of full length recombinant protein using IL-17E antibody (Biotin)