IL-17A antibody

• Catalog Number: orb345239
• Category: Antibodies
• Description: IL-17A antibody
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 20B4.G10.F5
• Tested applications: ELISA, FC, Multiplex Assay, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
• Concentration: 2.94 mg/mL
• Dilution range: ELISA: 1:10,000, FC: 1:500, WB: 1:1000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q62386
• NCBI: NP_034682.1
• Storage: Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rat anti-IL-17A antibody, rat anti-interleukin17A
• Note: For research use only
• Application notes: IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 um nitrocellulose using IL-17A antibody