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Catalog Number | orb429402 |
---|---|
Category | Proteins |
Description | Recombinant of IL 13 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Source | Escherichia Coli |
Biological Activity | ED50 range = 2-6 ng/mL as determined by the dose dependent proliferation of human TF-1 cells. Optimal concentration for individual application should be determined by a dose response assay. |
Storage | Stability: Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The protein (1mg/ml) was lyophilized with no additives. |
Alternative names | NC300, ALRH, BHR1, P600, IL-13. Read more... |
Note | For research use only |
Application notes | Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
39.0 kDa | |
Human IL-13 R alpha 2, His Tag (orb612134) is expressed from human 293 cells (HEK293). It contains AA Asp 27 - Arg 343 (Accession # Q14627-1). |
> 97% as determined by SDS-PAGE and HPLC. | |
12.3 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
12.5 kDa | |
E.Coli |
> 98% as determined by SDS-PAGE and HPLC. | |
12.6 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
12.3 kDa | |
E.Coli |