IKBKE Antibody

SKU: orb345377

Description

IKK E (phospho-T501) antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  IKKε pT501 antibody is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.
• Target: IKBKE
• Purity: Anti-IKKε pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity. This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe. Reactivity with non-phosphorylated IKKe is minimal. Cross reactivity with pT501 phosphorylated IKKe from mouse, rat or other species has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI

Quick Database Links

Gene Symbol

IKBKE

UniProt

Q14164

RefSeq:Q14164.1