IKB alpha (phospho-Y42) antibody

• Catalog Number: orb338946
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NFKBIA
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y42 of human IKB alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NFKBIA
• Entrez: 4792, 18035, 25493
• UniProt ID: Q9Z1E3, P25963, Q63746
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti IKBA antibody, anti MAD3 antibody, anti NFKBI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Western blot analysis of IKB alpha (Phospho-Y42) expression in K562 (A), Jurkat IL1b-treated (B), Raw264.7 LPS-treated (C), H9C2 LPS-treated (D) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 37 kD)

Immunohistochemical analysis of IKB alpha (Phospho-Y42) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.