NFKBIA (phospho-Tyr42) antibody

• Catalog Number: orb5520
• Category: Antibodies
• Description: NFKBIA (phospho-Tyr42) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Tyr42 (EE(p-Y)EQ)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: phospho-IKB alpha (Tyr42)
• UniProt ID: P25963
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: IKB alpha (phospho Y42); p-IKB alpha (phospho Y42)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ICC analysis of Hela cell using phospho-IKB alpha (Tyr42) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.

Western blot:Lane 1:HepG2 (Human) Cell;Lane 2:Hela (Human) Cell;Primary:Anti-phospho-IKB alpha (Tyr42) at 1:1000 dilution.

Western blot:Thymus(Mouse);Primary:Anti-phospho-IKB alpha (Tyr42) at 1:300 dilution.