IGSF3 Antibody

• Catalog Number: orb1256250
• Category: Antibodies
• Description: IGSF3 Antibody
• Target: IGSF3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-562 of human IGSF3 (NP_001007238.1).
• UniProt ID: O75054
• MW: Observed: 125kDa
• Tested applications: IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EWI-3, LCDD, V8, immunoglobulin superfamily member
• Note: For research use only

Western blot analysis of extracts of various cell lines, using IGSF3 Antibody (orb1256250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human prostate using IGSF3 antibody (orb1256250) (40x lens).