IGKC Antibody

• Catalog Number: orb1252752
• Category: Antibodies
• Description: IGKC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: L1C1
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human B-Lymphoma Cells were used as the immunogen for this Kappa light chain antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-2.0 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGKC
• UniProt ID: P01601, P01834
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-2.0 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin/paraffin human tonsil stained with Kappa light chain antibody (L1C1). Note cell membrane & cytoplasmic staining.

Western blot testing of Raji lysate with Kappa light chain antibody.

SDS-PAGE Analysis of Purified, BSA-Free Kappa Light Chain Antibody (clone L1C1). Confirmation of Integrity and Purity of the Antibody.