IGKC Antibody

• Catalog Number: orb1252750
• Category: Antibodies
• Description: IGKC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KLC709
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIHC (FrozenFFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGKC
• UniProt ID: P01601, P01834
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIHC (FrozenFFPE): 0.5-1.0 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil stained with anti-Kappa light chain antibody (KLC709).

Western blot testing of human spleen lysate with anti-Kappa light chain antibody (clone KLC709).