IGHM Antibody

• Catalog Number: orb1252754
• Category: Antibodies
• Description: IGHM Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IM260
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human IgM was used as the immunogen for this anti-IgM antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGHM
• UniProt ID: P01871
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil stained with anti-IgM antibody (IM260).

Western blot analysis of anti-IgM antibody and Raji cell lysate.

SDS-PAGE Analysis of Purified, BSA-Free Anti-IgM Antibody (clone IM260). Confirmation of Integrity and Purity of the Antibody.