IGHM Antibody

• Catalog Number: orb1252108
• Category: Antibodies
• Description: IGHM Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MuHC2
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human Mu heavy chain was used as the immunogen for this IgM antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Immunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IGHM
• UniProt ID: P01871
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Immunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IgM antibody and Raji cell lysate.

IHC analysis of FFPE human tonsil tissue and IgM antibody (MuHC2)