IGFBP6 Rabbit Polyclonal Antibody

SKU: orb5506

Description

IGFBP6 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGFBP6 (141-240/240aa)
• Target: IGFBP6
• Molecular Weight: 23 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IBP 6; IBP-6; IBP6; IBP6_HUMAN; IGF binding protein 6; IGF-binding protein 6; IGFBP 6; IGFBP-6; Insulin like growth factor binding protein 6; Insulin-like growth factor-binding protein 6.

Sample: Brain (Rat) Lysate at 40 ug, Intestine (Mouse) Lysate at 40 ug, Primary: Anti-IGFBP6 (orb5506) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 23 kD, Observed band size: 23 kD.

Sample: muscle (Mouse) Lysate at 40 ug, Primary: Anti-IGFBP6 (orb5506) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kD, Observed band size: 36 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated (orb5506) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IGFBP6

UniProt

P24592

References

Ozgecan Korkmaz Agaoglu, Ali Reha Agaoglu, Ozlem Ozmen, Ali Osman Turgut & Mustafa Saatci Expression and localization of insulin-like growth factor gene family members in the caprine ovarian and uterine tissues during different pregnancy stages Tropical Animal Health and Production, 55, 301 (2023)

PubMed: