IGFBP6 Rabbit Polyclonal Antibody

• Catalog Number: orb5506
• Category: Antibodies
• Description: IGFBP6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGFBP6 (141-240/240aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: IGFBP6
• UniProt ID: P24592
• RRID: AB_10935530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IBP 6; IBP-6; IBP6; IBP6_HUMAN; IGF binding protei
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ozgecan Korkmaz Agaoglu, Ali Reha Agaoglu, Ozlem Ozmen, Ali Osman Turgut & Mustafa Saatci Expression and localization of insulin-like growth factor gene family members in the caprine ovarian and uterine tissues during different pregnancy stages Tropical Animal Health and Production, 55, 301 (2023)

PubMed:

Sample: Brain (Rat) Lysate at 40 ug, Intestine (Mouse) Lysate at 40 ug, Primary: Anti-IGFBP6 (orb5506) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 23 kD, Observed band size: 23 kD.

Sample: muscle (Mouse) Lysate at 40 ug, Primary: Anti-IGFBP6 (orb5506) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23 kD, Observed band size: 36 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGFBP6 Polyclonal Antibody, Unconjugated (orb5506) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.