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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | IGFBP-7 |
| Tag | His Tag |
| MW | 27 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Asp 30 - Leu 282 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IGFBP7 Protein, His Tag [orb1173784]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 27.0 kDa after removal of the signal peptide. The apparent molecular mass of His-IGFBP7 is approximately 35-40 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgRecombinant Human Insulin-like growth factor-binding protein 7 (IGFBP7) (Active) [orb1785331]
Greater than 95% as determined by SDS-PAGE.
55.4 kDa
Mammalian cell
1 mg, 100 μg, 20 μgRecombinant Mouse IGFBP-7 Protein [orb3002302]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.2 KDa. Observed: 32-35 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human IGFBP-7 Protein [orb3002323]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 27.3 KDa. Observed: 30-35 KDa, reducing conditions
10 μg, 50 μg, 1 mg, 500 μg

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Human IGFBP-7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
IGFBP-7 Recombinant Protein (orb1227754)
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