IGF2R/M6PR Rabbit Polyclonal Antibody

SKU: orb500521

Description

IGF2R/M6PR Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation; Metabolism Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF2R/M6PR (701-800/2491aa)
• Target: IGF2R
• Molecular Weight: 300 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CI-MPR; CIMPR; CD222; CI-M6PR; M6P-R; M6P/IGF2R; MPR 300; MPR1; MPR300; MPRI; IGF-IIR; Igfr2; MPRI_BOVIN; IGF2R; CI Man-6-P receptor; M6PR; 300 kDa mannose 6-phosphate receptor (MPR 300); Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor (IGF-II receptor); M6P; MPRI_HUMAN; M6P/IGF2 receptor (M6P/IGF2R); MPRI_MOUSE;

Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-IGF2R/M6PR antibody (orb500521), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

IGF2R

UniProt

P11717