IGF2BP2 antibody

• Catalog Number: orb19766
• Category: Antibodies
• Description: Goat polyclonal antibody to IGF2BP2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 66.1; 61.8
• Target: IGF2BP2
• Entrez: 10644
• Protein Sequence: KQQEQKYPQGVASQR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti IGF2BP2 antibody, anti insulin-like growth fa
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 60-70kDa band observed in lysates of cell line HepG2 (calculated MW of 66.1kDa according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19766 (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing IGF2BP2 (RC205673) and probed with orb19766 (mock transfection in first lane), tested by Origene.