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Catalog Number | orb20564 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to IGF2BP1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 63.5; 48.6 |
Target | IMP1 / ZBP1 |
Entrez | 10642 |
Protein Sequence | EKVFAEHKISYSGQ |
RRID | AB_10753702 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti coding region determinant-binding protein ant Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3;). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
0.1 µg/ml staining of Caco-2 (A), (0.3 µg/ml) K562 (B), nuclear HepG2 (C) and (1 µg/ml) nuclear NIH3T3 (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
3.75 µg/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |