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IGF2BP1/IMP1

Catalog Number: orb20564

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb20564
CategoryAntibodies
DescriptionGoat polyclonal antibody to IGF2BP1
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, IF, IHC, WB
ReactivityBovine, Canine, Human, Mouse, Rat
Dilution rangeELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
ConjugationUnconjugated
MW63.5; 48.6
TargetIMP1 / ZBP1
Entrez10642
Protein SequenceEKVFAEHKISYSGQ
RRIDAB_10753702
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti coding region determinant-binding protein ant
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3;). Recommended concentration: 0.3-1 μg/ml.
Expiration Date12 months from date of receipt.
IGF2BP1/IMP1

0.1 µg/ml staining of Caco-2 (A), (0.3 µg/ml) K562 (B), nuclear HepG2 (C) and (1 µg/ml) nuclear NIH3T3 (D) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

IGF2BP1/IMP1

3.75 µg/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

IGF2BP1/IMP1

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

IGF2BP1/IMP1

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

IGF2BP1/IMP1

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.