IGF2 Rabbit Polyclonal Antibody

• Catalog Number: orb10887
• Category: Antibodies
• Description: IGF2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF2 (25-51/180aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 7.4 kDa
• Target: IGF2
• UniProt ID: P01344
• RRID: AB_10749402
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: IGF2_HUMAN; Insulin-like growth factor II; IGF-II;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

R Hernández, GG Castro, FM Rodríguez Insulin-like growth factors in cat placenta Analecta Veterinaria, (2025)

PubMed:

Sant'Ana, Fjf et al. Immunohistochemical expression of growth factors in the follicular wall of normal and cystic ovaries of sows Reprod Domest Anim, 50, 327-332 (2015)

PubMed: 25676567

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-IGF II antibody (orb10887), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IGF II) Polyclonal Antibody, Unconjugated (orb10887) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGF II Polyclonal Antibody, Unconjugated (orb10887) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.