IGF1R Rabbit Polyclonal Antibody

SKU: orb10881

Description

IGF1R Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IGF-1R alpha chain (251-350/1367aa)
• Target: IGF1R
• Molecular Weight: 78/150 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Insulin-like growth factor 1 receptor; CD221; CD221 antigen; IGF1 Receptor; IGF 1 receptor; IGF 1R; IGF I receptor; IGFIRC; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; Insulin-like growth factor 1 receptor beta chain; IGF 1 receptor; IGF-1R; IGF I receptor; IGF-I receptor; IGF-IR; IGF1R_HUMAN.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-IGF1R antibody (orb10881), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, catalog number: orb10881, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Quick Database Links

Gene Symbol

IGF1R

UniProt

P08069

References

Hu, Wei et al. MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene Growth Factors, 32, 27-33 (2014)

PubMed: 24294913