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IGBP1 Rabbit Polyclonal Antibody

Catalog Number: orb10348

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb10348
CategoryAntibodies
DescriptionIGBP1 Rabbit Polyclonal Antibody
TargetIGBP1
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Rat
Predicted ReactivityBovine, Guinea pig, Mouse, Porcine
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human Immunoglobulin binding protein 1 (151-250/339aa)
UniProt IDP78318
RRIDAB_10749646
MW44 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesalpha 4; B cell signal transduction molecule alpha
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NoteFor research use only
IGBP1 Rabbit Polyclonal Antibody

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-IGBP1 antibody (orb10348), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

IGBP1 Rabbit Polyclonal Antibody

Tissue/Cell: Rat lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IGBP1 Polyclonal Antibody, Unconjugated (orb10348) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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