Ig Kappa Light Chain Antibody (PE)

• Catalog Number: orb179919
• Category: Antibodies
• Description: Anti-Hu Ig Kappa Light Chain PE
• Clonality: Monoclonal
• Clone Number: TB28-2
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Human IgG-kappa myeloma protein
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: Ig Kappa Light Chain
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Immunoglobulin kappa, Igk
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human CD19 positive B cells using anti-human Ig Kappa Light Chain (TB28-2) PE (c = 5 µg/ml) and anti-human Ig Lambda Light Chain (1-155-2) FITC (c = 5 µg/ml) antibodies.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human Ig Lambda Kappa Chain positive B cells (red-filled) from Ig Kappa Light Chain negative B cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig Kappa Light Chain (TB28-2) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).