IFNGR2 Rabbit Polyclonal Antibody

• Catalog Number: orb5501
• Category: Antibodies
• Description: IFNGR2 Rabbit Polyclonal Antibody
• Target: IFNGR2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human IFNGR2 (241-337/337aa)
• UniProt ID: P38484
• RRID: AB_10935847
• MW: 36 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFN gamma Receptor beta; AF 1; AF1; IFGR 2; IFGR2;
• Note: For research use only

References

Gabriel Natura 1, Enrique Vazquez 1, Frank Richter 1, Gisela Segond von Banchet 1, Matthias Ebbinghaus 1, Andrea Ebersberger 1, Christian König 1, Jakob Maltritz 1, Mieczyslaw Gajda 2, Christoph Schmidt-Hieber 1, Hans-Georg Schaible Antinociceptive interactions between excitatory interferon-γ and interleukin-17 in sensory neurons Brain Behav Immun, (2024)

PubMed: 39566665

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human U937 cell lysates, Primary: Anti-IFNGR2 (orb5501) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFNGR2 Polyclonal Antibody, Unconjugated (orb5501) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.