IFNGR2 Rabbit Polyclonal Antibody

SKU: orb5501

Description

IFNGR2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IFNGR2 (241-337/337aa)
• Target: IFNGR2
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

IFN gamma Receptor beta; AF 1; AF1; IFGR 2; IFGR2; IFNGR 2; IFNGT 1; IFNGT1; Interferon gamma receptor 2; Interferon gamma receptor accessory factor 1; Interferon gamma receptor beta chain; Interferon gamma receptor chain 2; Interferon gamma transducer 1; INGR2_HUMAN; gamma receptor 2; IFN-gamma receptor 2; IFN-gamma-R2; Interferon gamma receptor accessory factor 1; AF 1; AF-1; AF1; Interferon gamma transducer 1.

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human U937 cell lysates, Primary: Anti-IFNGR2 (orb5501) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 35 kD.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFNGR2 Polyclonal Antibody, Unconjugated (orb5501) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IFNGR2

UniProt

P38484

References

Gabriel Natura 1, Enrique Vazquez 1, Frank Richter 1, Gisela Segond von Banchet 1, Matthias Ebbinghaus 1, Andrea Ebersberger 1, Christian König 1, Jakob Maltritz 1, Mieczyslaw Gajda 2, Christoph Schmidt-Hieber 1, Hans-Georg Schaible Antinociceptive interactions between excitatory interferon-γ and interleukin-17 in sensory neurons Brain Behav Immun, (2024)

PubMed: 39566665