IFNB1 Antibody

• Catalog Number: orb1272621
• Category: Antibodies
• Description: IFNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: E.coli derived Recombinant Human IFN-β
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: IFNB1
• UniProt ID: P01574
• NCBI: P01574
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast i
• Note: For research use only
• Application notes: ELISA:Sandwich:To detect Human IFN-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with BIorbyt’s Polyclonal Biotinylated Anti-Human IFN-beta (38-219) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta. Western Blot:: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human IFN-beta (5.00μg/mL), a concentration of 2.5 μg/mL of this antibody is required.
• Expiration Date: 12 months from date of receipt.

To detect Human IFN-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-beta (orb1272677) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta.

To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.