IFNB1 Antibody

• Catalog Number: orb1239454
• Category: Antibodies
• Description: IFNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-IFN-beta antibody (orb1239454) was raised against a peptide corresponding to 17 amino acids near the center of human IFN-beta. The immunogen is located within amino acids 110-160 of IFN-beta.
• Concentration: 1 mg/mL
• Dilution range: WB: 4-10 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 22kDObserved: 22kD.
• Target: IFNB1
• UniProt ID: P01574
• NCBI: AAC41702
• Storage: IFN-beta antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IFN-beta Antibody: IFB, IFF, IFNB, IFB, Interferon
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Mouse A20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (Lane 1: 5 µg/mL and Lane 2: 10 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (4 µg/mL), IFN-beta, (5 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Multiple Tissues and Recombinant Protein. Loading: 15 µg of lysates per lane. Antibodies: IFN-beta orb1239454 (4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of IFN-beta in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (orb1239454) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IFN-beta in Human Liver Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with orb1239454 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of IFN-beta in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (orb1239454) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of IFN-beta in Mouse Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling IFN-beta with orb1239454 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).