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| Catalog Number | orb1272620 |
|---|---|
| Category | Antibodies |
| Description | IFN-lamda2 Antibody |
| Target | IFNL2 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Purification | Human IFN-lamda2 specific antibody was purified by affinity chromatography employing immobilized Human IFN-lamda2 matrix. |
| Immunogen | E.coli derived Recombinant Human IFN-λ2 |
| UniProt ID | Q8IZJ0 |
| Tested applications | ELISA, IHC, WB |
| Application notes | ELISA:Sandwich:To detect Human IFN-lamda2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IFN-lamda2 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-lamda2. Western Blot:: To detect Human IFN-lamda2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-lamda2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Immunohistochemistry:This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/mL overnight at 4˚C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | IL28A, IL-28A, IL28A, ZCYTO20, Interferon lambda-2 Read more... |
| Note | For research use only |
| NCBI | Q8IZJ0 |
| Expiration Date | 12 months from date of receipt. |

To detect Human IFN-λ2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-λ2 (orb1272723) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-λ2.

To detect Human IFN-λ2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-λ2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1.0 mg/ml overnight at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen and proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
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