IFN gamma Rabbit Polyclonal Antibody

SKU: orb10877

Description

IFN gamma Rabbit Polyclonal Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: FC, ICC
• Dilution Range: Flow-Cyt=1ug/Test, ICC/IF=1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IFN Gamma (81-166/166aa)
• Target: IFNG
• Molecular Weight: 20, 25 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IFG; IFI; IMD69; IFN-g; If2f; IFNG_HUMAN; IFNG; IFN-gamma; Immune interferon; IFNG_MOUSE;

Sample: HL-60 Cell (Human) Lysate at 40 ug, Primary: Anti-IFN gamma (orb10877) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 16 kD.

Sample: Molt-4 Cell (Human) Lysate at 40 ug, Primary: Anti-IFN gamma (orb10877) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 16 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated (orb10877) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IFNG

UniProt

P01579