IDH3A Antibody

• Catalog Number: orb1249285
• Category: Antibodies
• Description: IDH3A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: DFTEEICRRVKDLD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: IDH3A
• UniProt ID: P50213
• NCBI: NP_005521.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249285 (0.1 ug/ml) staining of Human Lymph Nodes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249285 (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249285 (0.3 ug/ml) staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249285 (3.75 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.