IDH2 Antibody

• Catalog Number: orb312128
• Category: Antibodies
• Description: IDH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunohistochemistry (Frozen Section)|0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 50909 MW
• UniProt ID: P48735
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Isocitrate dehydrogenase [NADP], mitochondrial;IDH
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in immunocytochemical section of SW480 cell.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of SiHa cells using anti-IDH2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of IDH2 using anti-IDH2 antibody.Lane 1:Rat Cardiac Muscle Tissue;2:Rat Liver Tissue;3:NIH3T3 Cell;4:SW620 Cell;5:HELA Cell;6:MCF-7 Cell;7:22RV1 Cell.

IF analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in immunocytochemical section of NRK cells.

IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.

IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in paraffin-embedded section of Mouse Intestine Tissue.

IHC analysis of IDH2 using anti-IDH2 antibody. IDH2 was detected in paraffin-embedded section of Rat Intestine Tissue.

IHC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in frozen section of rat small intestine tissue.

IHC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in frozen section of human placenta tissue.

IHC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in frozen section of mouse small intestine tissue.

IHC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in immunocytochemical section of A549 cell.

IHC analysis of IDH2 using anti-IDH2 antibody.IDH2 was detected in immunocytochemical section of Hep1-6 cell.