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    IDH2 Antibody

    Catalog Number: orb1256539

    DispatchUsually dispatched within 5-10 working days
    $ 731.00
    Catalog Numberorb1256539
    CategoryAntibodies
    DescriptionIDH2 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsChIP, IF, IHC, IP, WB
    ReactivityHuman, Mouse, Rat
    IsotypeIgG
    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
    Concentrationbatch dependent
    Dilution rangeWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50IP: 1:50 - 1:200ChIP: 1:20 - 1:100
    Form/AppearanceLiquid
    ConjugationUnconjugated
    MWObserved: 51kDa
    TargetIDH2
    UniProt IDP48735
    StorageStore at -20˚°C. Avoid freeze / thaw cycles.
    Buffer/PreservativesLiquid
    Alternative namesIDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2
    Read more...
    NoteFor research use only
    Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50IP: 1:50 - 1:200ChIP: 1:20 - 1:100
    Expiration Date12 months from date of receipt.
    IDH2 Antibody

    Western blot analysis of extracts of various cell lines, using IDH2 antibody (orb1256539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

    IDH2 Antibody

    Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 antibody (orb1256539) at dilution of 1:100 (40x lens).

    IDH2 Antibody

    Immunofluorescence analysis of A-549 cells using IDH2 antibody (orb1256539). Blue: DAPI for nuclear staining.

    IDH2 Antibody

    Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 1 ug IDH2 antibody (orb1256539). Western blot was performed from the immunoprecipitate using IDH2 antibody (orb1256539) at a dilution of 1:1000.

    IDH2 Antibody

    Chromatin immunoprecipitation of extracts of 293T cell line, using IDH2 antibody (orb1256539) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    • IDH2 Antibody [orb312128]

      FC,  ICC,  IF,  IHC,  IHC-Fr,  WB

      Hamster

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      10 μg, 100 μg
    • IDH2 Antibody (monoclonal, 2H4) [orb692220]

      IHC,  WB

      Human, Mouse, Rat

      Mouse

      Monoclonal

      Unconjugated

      10 μg, 100 μg
    • IDH2 antibody [orb55864]

      IHC-P,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μg, 200 μg
    • IDH2 Antibody (monoclonal, 6B13) [orb692221]

      FC,  ICC,  IF,  IHC,  WB

      Human, Mouse, Rat

      Mouse

      Monoclonal

      Unconjugated

      10 μg, 100 μg
    • IDH2 Antibody [orb622348]

      FC,  IHC,  WB

      Human, Mouse, Rat

      Rabbit

      Recombinant

      Unconjugated

      50 μl, 100 μl
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