IDH2 Antibody

• Catalog Number: orb1256539
• Category: Antibodies
• Description: IDH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50IP: 1:50 - 1:200ChIP: 1:20 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 51kDa
• Target: IDH2
• UniProt ID: P48735
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50IP: 1:50 - 1:200ChIP: 1:20 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IDH2 antibody (orb1256539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 antibody (orb1256539) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using IDH2 antibody (orb1256539). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 1 ug IDH2 antibody (orb1256539). Western blot was performed from the immunoprecipitate using IDH2 antibody (orb1256539) at a dilution of 1:1000.

Chromatin immunoprecipitation of extracts of 293T cell line, using IDH2 antibody (orb1256539) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.