IDH2 Antibody

SKU: orb1256539

Description

IDH2 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50IP: 1:50 - 1:200ChIP: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
• Target: IDH2
• Molecular Weight: Observed: 51kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

IDH2 Antibody: IDH, IDP, IDHM, IDPM, ICD-M, D2HGA2, mNADP-IDHICD-M, IDH

Western blot analysis of extracts of various cell lines, using IDH2 antibody (orb1256539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 antibody (orb1256539) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A-549 cells using IDH2 antibody (orb1256539). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 1 ug IDH2 antibody (orb1256539). Western blot was performed from the immunoprecipitate using IDH2 antibody (orb1256539) at a dilution of 1:1000.

Chromatin immunoprecipitation of extracts of 293T cell line, using IDH2 antibody (orb1256539) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Quick Database Links

Gene Symbol

IDH2

UniProt

P48735