IDH2 Antibody

• Catalog Number: orb1523620
• Category: Antibodies
• Description: IDH2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 402 and 452
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:2,500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: IDH2
• UniProt ID: P48735
• NCBI: NP_002159.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: IDH; oxalosuccinate decarboxylase; NADP(+)-specifi
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse IDH2 by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody orb1523620 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human IDH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-IDH2 antibody orb1523620 used for IP at 6 µg/mg lysate.

Detection of mouse IDH2 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human IDH2 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma (left) and lung cancer (right). Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human IDH2 by immunocytochemistry.Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-IDH2 (Cat. No. orb1523620) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100.