ICMT Rabbit Polyclonal Antibody

• Catalog Number: orb2269
• Category: Antibodies
• Description: ICMT Rabbit Polyclonal Antibody
• Target: ICMT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CMT/Icmt (211-278/284aa)
• UniProt ID: O60725
• MW: 32 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CMT; HSTE14; Icmt; ICMT_HUMAN; Isoprenylcysteine c
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ICMT) Polyclonal Antibody, Unconjugated (orb2269) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human pancreatic carcinoma (BXPC-3), 4% Paraformaldehyde-fixed, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ICMT Polyclonal Antibody, Unconjugated (orb2269) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated (orb868590) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.