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Catalog Number | orb19347 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ICAM5 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Reactivity | Bovine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 0.05 μg/ml, IHC-P: 2-4µg/ml |
Conjugation | Unconjugated |
MW | 97.1 |
Target | ICAM5 precursor |
Entrez | 7087 |
Protein Sequence | ESPAEGEVFAIQ |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ICAM5 antibody, anti intercellular adhesion m Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb19347 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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