ICAM5 antibody

• Catalog Number: orb101328
• Category: Tools
• Description: ICAM5 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ICAM5 (501-600/924 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=0.2ug/test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 97 kDa
• Target: ICAM5
• UniProt ID: Q9UMF0
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: ICAM5_HUMAN; ICAM5 precursor; Intercellular adhesi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- ICAM5 (orb101328) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 97 kD Observed band size: 167 kD

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ICAM5 antibody (orb101328) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 0.2μg /test. Protocol