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Catalog Number | orb315542 |
---|---|
Category | Proteins |
Description | ICAM1 protein |
Species/Host | E. coli |
Tested applications | ELISA, SDS-PAGE, WB |
Reactivity | Human |
Concentration | 1 mg/ml |
Form/Appearance | 20mM Tris-HCl, 50mM NaCl, 1M Urea,1mM DTT, pH 7.5 |
Purity | > 90.0% as determined by 12% SDS-PAGE |
Conjugation | Unconjugated |
MW | 23.8 kDa |
Target | ICAM1 |
Protein Sequence | QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFV |
NCBI | 000201 |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Alternative names | BB2 protein, CD 54 protein, ICAM 1 protein, ICAM-1 Read more... |
Note | For research use only |
Application notes | N-His6 |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of ICAM1 protein
Unconjugated | |
98% | |
75.7 kDa | |
Human ICAM-1, Fc Tag (orb257553) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # AAH15969). |
Unconjugated | |
95% | |
26.3 kDa | |
Human Angiopoietin-2 (275-496), His Tag (orb257185) is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496 (Accession # AAI26201.1). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 75.7 kDa after removal of the signal peptide.The apparent molecular mass of ICAM-1-mFc is approximately 90-120 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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