iASPP Antibody

• Catalog Number: orb1527880
• Category: Antibodies
• Description: iASPP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 75 and 125
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: iASPP
• UniProt ID: Q8WUF5
• NCBI: NP_006654.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: protein iASPP; IASPP; relA-associated inhibitor; r
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse iASPP by western blot. Samples: Whole cell lysate from HEK293T (50, 15, 5 µg) , HeLa (50, 15 µg) , and mouse NIH 3T3 (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody orb1527880 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human iASPP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-iASPP antibody orb1527880 used for IP at 6 µg per reaction.