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HUS1 Antibody
Catalog Number: orb1257506
| Catalog Number | orb1257506 |
|---|---|
| Category | Antibodies |
| Description | HUS1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IP, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 35kDa |
| Target | HUS1 |
| UniProt ID | O60921 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | Checkpoint protein HUS1, hHUS1, HUS1 Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HUS1 antibody (orb1257506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of U2OS cells using HUS1 antibody (orb1257506). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using HUS1 antibody (orb1257506). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug HUS1 antibody (orb1257506). Western blot was performed from the immunoprecipitate using HUS1 antibody (orb1257506) at a dilution of 1:1000.
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- Item 1 of 5
- Item 1 of 3
- Item 1 of 3
Anti-HUS1 Antibody [orb378112]
IF, IH, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl - Item 1 of 2
