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Catalog Number | orb392708 |
---|---|
Category | Proteins |
Description | Recombinant human VSIG4 protein is produced by our mammalian expression system and the target gene encoding Arg20-Val284 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | VSIG4 |
Entrez | 11326 |
UniProt ID | Q9Y279 |
Protein Sequence | RPILEVPESV TGPWKGDVNL PCTYDPLQGY TQVLVKWLVQ RGSDPVTIFL RDSSGDHIQQ AKYQGRLHVS HKVPGDVSLQ LSTLEMDDRS HYTCEVTWQT PDGNQVVRDK ITELRVQKLS VSKPTVTTGS GYGFTVPQGM RISLQCQARG SPPISYIWYK QQTNNQEPIK VATLSTLLFK PAVIADSGSY FCTAKGQVGS EQHSDIVKFV VKDSSKLLKT KTEAPTTMTY PLKATSTVKQ SWDWTTDMDG YLGETSAGPG KSLPVDDIEG RMDEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSREEMTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | CRIg; Z39IG; V-set and immunoglobulin domain-conta Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
30.1 kDa | |
Human VSIG4, His Tag (orb257931) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 283 (Accession # AAH10525). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.3 kDa after removal of the signal peptide. The apparent molecular mass of VSIG4-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Human | |
31.25 pg/mL-2000 pg/mL | |
7.81 pg/mL |
Greater than 95% as determined by SDS-PAGE. | |
30.6 kDa | |
Mammalian cell |
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