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Catalog Number | orb392707 |
---|---|
Category | Proteins |
Description | Recombinant human VSIG4 protein is produced by our Mammalian expression system and the target gene encoding Arg20-Pro283 is expressed with a 6His tag at the C-terminus. |
Target | VSIG4 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | RPILEVPESV TGPWKGDVNL PCTYDPLQGY TQVLVKWLVQ RGSDPVTIFL RDSSGDHIQQ AKYQGRLHVS HKVPGDVSLQ LSTLEMDDRS HYTCEVTWQT PDGNQVVRDK ITELRVQKLS VSKPTVTTGS GYGFTVPQGM RISLQCQARG SPPISYIWYK QQTNNQEPIK VATLSTLLFK PAVIADSGSY FCTAKGQVGS EQHSDIVKFV VKDSSKLLKT KTEAPTTMTY PLKATSTVKQ SWDWTTDMDG YLGETSAGPG KSLPVDHHHH HH |
UniProt ID | Q9Y279 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | CRIg; Z39IG; V-set and immunoglobulin domain-conta Read more... |
Note | For research use only |
Entrez | 11326 |
Greater than 95% as determined by SDS-PAGE. | |
30.6 kDa | |
Mammalian cell |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.3 kDa after removal of the signal peptide. The apparent molecular mass of VSIG4-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Human | |
31.25 pg/mL-2000 pg/mL | |
7.81 pg/mL |