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Catalog Number | orb392704 |
---|---|
Category | Proteins |
Description | Recombinant human VNN2 protein is produced by our Mammalian expression system and the target gene encoding Gln23-Ser492 is expressed with a 6His tag at the C-terminus. |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | QDSFIAAVYE HAVILPNKTE TPVSQEDALN LMNENIDILE TAIKQAAEQG ARIIVTPEDA LYGWKFTRET VFPYLEDIPD PQVNWIPCQD PHRFGHTPVQ ARLSCLAKDN SIYVLANLGD KKPCNSRDST CPPNGYFQYN TNVVYNTEGK LVARYHKYHL YSEPQFNVPE KPELVTFNTA FGRFGIFTCF DIFFYDPGVT LVKDFHVDTI LFPTAWMNVL PLLTAIEFHS AWAMGMGVNL LVANTHHVSL NMTGSGIYAP NGPKVYHYDM KTELGKLLLS EVDSHPLSSL AYPTAVNWNA YATTIKPFPV QKNTFRGFIS RDGFNFTELF ENAGNLTVCQ KELCCHLSYR MLQKEENEVY VLGAFTGLHG RRRREYWQVC TMLKCKTTNL TTCGRPVETA STRFEMFSLS GTFGTEYVFP EVLLTEIHLS PGKFEVLKDG RLVNKNGSSG PILTVSLFGR WYTKDSLYSS VDHHHHHH |
UniProt ID | O95498 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Vascular non-inflammatory molecule 2; Vanin-2; Gly Read more... |
Research Area | Epigenetics |
Note | For research use only |
Entrez | 8875 |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
44.9 kDa | |
E.Coli |
54.6 kDa (predicted); 55-60 kDa (reducing condition, due to glycosylation) |
>98%, determined by SDS-PAGE | |
This protein contains the human VNN2 isoform 1 (NP_0046562) without the propeptide (Met 1-Ser 492) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |