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Catalog Number | orb429211 |
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Category | Proteins |
Description | Recombinant of human VNN1 protein |
Form/Appearance | Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | VNN1 was filtered (0.4 µm) and lyophilized in 20mM Tris buffer, 50mM NaCl and 0.1% amisoft CS-22, pH 7.5. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MKHHHHHHASQDTFTAAVYE HAAILPNATL TPVSREEALA LMNRNLDILE GAITSAADQG AHIIVTPEDA IYGWNFNRDS LYPYLEDIPD PEVNWIPCNN RNRFGQTPVQ ERLSCLAKNN SIYVVANIGD KKPCDTSDPQ CPPDGRYQYN TDVVFDSQGK LVARYHKQNL FMGENQFNVP KEPEIVTFNT TFGSFGIFTC FDILFHDPAV TLVKDFHVDT IVFPTAWMNV LPHLSAVEFH SAWAMGMRVN FLASNIHYPS KKMTGSGIYA PNSSRAFHYD MKTEEGKLLL SQLDSHPSHS AVVNWTSYAS SIEALSSGNK EFKGTVFFDE FTFVKLTGVA GNYTVCQKDL CCHLSYKMSE NIPNEVYALG AFDGLHTVEG RYYLQICTLL KCKTTNLNTC GDSAETASTR FEMFSLSGTF GTQYVFPEVL LSENQLAPGE FQVSTDGRLF SLKPTSGPVL TVTLFGRLYE KDWASNASSG |
Application notes | Recombinant & Natural Proteins |
Source | Escherichia Coli |
Solubility (25°C) | It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. VNN1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
Storage | Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C |
Alternative names | Vanin 1, Vascular Non-Inflammatory Molecule 1, Pan Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
> 95% by SDS-PAGE. | |
Recombinant Human Vanin-1/VNN1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln22-Ser490) of human Vanin-1/VNN1 (Accession #NP_004657.2) fused with a 6��His Tag at the C-terminus. |
98.10% | |
53.7 kDa (predicted); 70-75 kDa (reducing condition, due to glycosylation) |
>97%, determined by SDS-PAGE | |
This protein contains the human VNN1 (NP_0046572) without the propeptide (Met 1-Ser 490) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |