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Catalog Number | orb392703 |
---|---|
Category | Proteins |
Description | Recombinant human VNN1 protein is produced by our Mammalian expression system and the target gene encoding Gln22-Ser490 is expressed with a 6His tag at the C-terminus. |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | QDTFTAAVYE HAAILPNATL TPVSREEALA LMNRNLDILE GAITSAADQG AHIIVTPEDA IYGWNFNRDS LYPYLEDIPN PEVNWIPCNN RNRFGQTPVQ ERLSCLAKNN SIYVVANIGD KKPCDTSDPQ CPPDGRYQYN TDVVFDSQGK LVARYHKQNL FMGENQFNVP KEPEIVTFNT TFGSFGIFTC FDILFHDPAV TLVKDFHVDT IVFPTAWMNV LPHLSAVEFH SAWAMGMRVN FLASNIHYPS KKMTGSGIYA PNSSRAFHYD MKTEEGKLLL SQLDSHPSHS AVVNWTSYAS SIEALSSGNK EFKGTVFFDE FTFVKLTGVA GNYTVCQKDL CCHLSYKMSE NIPNEVYALG AFDGLHTVEG RYYLQICTLL KCKTTNLNTC GDSAETASTR FEMFSLSGTF GTQYVFPEVL LSENQLAPGE FQVSTDGRLF SLKPTSGPVL TVTLFGRLYE KDWASNASSV DHHHHHH |
UniProt ID | O95497 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Pantetheinase; Pantetheine hydrolase; Tiff66; Vasc Read more... |
Research Area | Epigenetics |
Note | For research use only |
Entrez | 8876 |
Expiration Date | 6 months from date of receipt. |
> 95% by SDS-PAGE. | |
Recombinant Human Vanin-1/VNN1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln22-Ser490) of human Vanin-1/VNN1 (Accession #NP_004657.2) fused with a 6��His Tag at the C-terminus. |
Greater than 95.0% as determined by SDS-PAGE. | |
Escherichia Coli |
98.10% | |
53.7 kDa (predicted); 70-75 kDa (reducing condition, due to glycosylation) |
>97%, determined by SDS-PAGE | |
This protein contains the human VNN1 (NP_0046572) without the propeptide (Met 1-Ser 490) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |