Human VEGF121 Protein

• Catalog Number: orb257918
• Category: Proteins
• Description: Human VEGF121 Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 14.1 kDa
• Target: VEGF121
• UniProt ID: P15692
• Source: Human VEGF121 Protein, premium grade (orb257918) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Human VEGF121 Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested).
• Expression Region: Ala 27 - Arg 147
• Endotoxins: 0.1 EU per μg
• NCBI: NP_001165099.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 15-16 kDa and 17-20 kDa under reducing (R) condition, and 30-40 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Stafford, Ryan L. et al. In vitro Fab display: a cell-free system for IgG discovery Protein Eng Des Sel, 27, 97-109 (2014)

PubMed: 24586053

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Immobilized Human VEGF121 Protein, premium grade (Cat. No. orb257918) at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested).