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Human VEGF121 Protein
Description
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | VEGF121 |
| Reactivity | Human |
| Tag | Native |
| Molecular Weight | 14.1 kDa |
| Expression Region | Ala 27 - Arg 147 |
| Purity | 95% |
| Endotoxins | 0.1 EU per μg |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
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−Human VEGF121 Protein, hFc Tag [orb2321385]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgHuman VEGFA Protein [orb2697487]
>92%, determined by SDS-PAGE
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells.
1 mg, 100 μgHuman VEGFA Protein, His Tag [orb2697421]
>95%, determined by SDS-PAGE
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells.
50 μg, 20 μgHuman VEGFA Protein [orb2701983]
>(55.6 + 41.6)%, determined by SDS-PAGE
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells.
20 μg, 10 μg

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SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Immobilized Human VEGF121 Protein, premium grade (Cat. No. orb257918) at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested).
Quick Database Links
UniProt Details
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Protocol Information
Stafford, Ryan L. et al. In vitro Fab display: a cell-free system for IgG discovery Protein Eng Des Sel, 27, 97-109 (2014)
Human VEGF121 Protein (orb257918)
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