Human USP14 protein

SKU: orb392688

Description

Recombinant human USP14 protein is produced by our E. coli expression system and the target gene encoding Asp91-Gln494 is expressed with a 6His tag at the N-terminus.

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Reactivity: Human
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRLQEEIT KQSPTLQRNA LYIKSSKISR LPAYLTIQMV RFFYKEKESV NAKVLKDVKF PLMLDMYELC TPELQEKMVS FRSKFKDLED KKVNQQPNTS DKKSSPQKEV KYEPFSFADD IGSNNCGYYD LQAVLTHQGR SSSSGHYVSW VKRKQDEWIK FDDDKVSIVT PEDILRLSGG GDWHIAYVLL YGPRRVEIME EESEQ
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 100mM NaCl, 20% Glycerol, pH 8.0.
• Disclaimer: For research use only

Alternative Names

TGT; Ubiquitin carboxyl-terminal hydrolase 14; Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14

Quick Database Links

UniProt

P54578

NCBI Gene ID

9097