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Catalog Number | orb392656 |
---|---|
Category | Proteins |
Description | Recombinant human UBE2D1 protein is produced by our E. coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerin pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | UBE2D1 |
Entrez | 7321 |
UniProt ID | P51668 |
Protein Sequence | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMALK RIQKELSDLQ RDPPAHCSAG PVGDDLFHWQ ATIMGPPDSA YQGGVFFLTV HFPTDYPFKP PKIAFTTKIY HPNINSNGSI CLDILRSQWS PALTVSKVLL SICSLLCDPN PDDPLVPDIA QIYKSDKEKY NRHAREWTQK YAM |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerin pH 7.5. |
Alternative names | SFT; UBC5A; UBCH5; UBCH5A; Ubiquitin-conjugating e Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Greater than 95.0% as determined by SDS-PAGE. | |
Escherichia Coli |
98.00% | |
40 KDa (reducing condition) |