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Catalog Number | orb392650 |
---|---|
Category | Proteins |
Description | Recombinant human UBB protein is produced by our E. coli expression system and the target gene encoding Met153-Gly228 is expressed with a GST, 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | UBB |
Entrez | 7314 |
UniProt ID | P0CG47 |
Protein Sequence | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKSPM GYRGSMQIFV KTLTGKTITL EVEPSDTIEN VKAKIQDKEG IPPDQQRLIF AGKQLEDGRT LSDYNIQKES TLHLVLRLRG G |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | Polyubiquitin-B Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
27.0 kDa | |
Human UCH-L3, His Tag (orb348793) is expressed from E. coli cells. It contains AA Glu 2 - Ala 230 (Accession # P15374-1). |
ELISA, ICC, WB | |
Bovine, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
ATTO-390 |
ELISA, ICC, WB | |
Bovine, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
ATTO-488 |
ELISA, ICC, WB | |
Bovine, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
ATTO-594 |
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