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Catalog Number | orb392648 |
---|---|
Category | Proteins |
Description | Recombinant human UBA5 protein is produced by our E. coli expression system and the target gene encoding Met1-Met404 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 10% glycerol, 50mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | UBA5 |
Entrez | 79876 |
UniProt ID | Q9GZZ9 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MAESVERLQQ RVQELERELA QERSLQVPRS GDGGGGRVRI EKMSSEVVDS NPYSRLMALK RMGIVSDYEK IRTFAVAIVG VGGVGSVTAE MLTRCGIGKL LLFDYDKVEL ANMNRLFFQP HQAGLSKVQA AEHTLRNINP DVLFEVHNYN ITTVENFQHF MDRISNGGLE EGKPVDLVLS CVDNFEARMT INTACNELGQ TWMESGVSEN AVSGHIQLII PGESACFACA PPLVVAANID EKTLKREGVC AASLPTTMGV VAGILVQNVL KFLLNFGTVS FYLGYNAMQD FFPTMSMKPN PQCDDRNCRK QQEEYKKKVA ALPKQEVIQE EEEIIHEDNE WGIELVSEVS EEELKNFSGP VPDLPEGITV AYTIPKKQED SVTELTVEDS GESLEDLMAK MKNM |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 10% glycerol, 50mM NaCl, pH 8.0. |
Alternative names | UBE1DC1; Ubiquitin-like modifier-activating enzyme Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
44.3 kDa | |
E.Coli |
ELISA, WB | |
Greater than 90% by SDS-PAGE gel analyses | |
43.6 kDa | |
E.Coli |
98.00% | |
14&40-60 KDa, reducing conditions |
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