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Catalog Number | orb392640 |
---|---|
Category | Proteins |
Description | Recombinant human TUBB4A protein is produced by our E. coli expression system and the target gene encoding Met1-Ala444 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | TUBB4A |
Entrez | 10382 |
UniProt ID | P04350 |
Protein Sequence | MNHKVHHHHH HMREIVHLQA GQCGNQIGAK FWEVISDEHG IDPTGTYHGD SDLQLERINV YYNEATGGNY VPRAVLVDLE PGTMDSVRSG PFGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDAVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEF PDRIMNTFSV VPSPKVSDTV VEPYNATLSV HQLVENTDET YCIDNEALYD ICFRTLKLTT PTYGDLNHLV SATMSGVTTC LRFPGQLNAD LRKLAVNMVP FPRLHFFMPG FAPLTSRGSQ QYRALTVPEL TQQMFDAKNM MAACDPRHGR YLTVAAVFRG RMSMKEVDEQ MLSVQSKNSS YFVEWIPNNV KTAVCDIPPR GLKMAATFIG NSTAIQELFK RISEQFTAMF RRKAFLHWYT GEGMDEMEFT EAESNMNDLV SEYQQYQDAT AEEGEFEEEA EEEVAVDLQS R |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | TUBB4; TUBB5; Tubulin beta-4A chain; Tubulin 5 bet Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
53.6 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
51.6 kDa | |
Yeast |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
48.7 kDa | |
E.Coli |
98.00% | |
58 KDa, reducing conditions |
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