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Human TUBB4A protein

SKU: orb392640

Description

Recombinant human TUBB4A protein is produced by our E. coli expression system and the target gene encoding Met1-Ala444 is expressed with a 6His tag at the N-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMNHKVHHHHH HMREIVHLQA GQCGNQIGAK FWEVISDEHG IDPTGTYHGD SDLQLERINV YYNEATGGNY VPRAVLVDLE PGTMDSVRSG PFGQIFRPDN FVFGQSGAGN NWAKGHYTEG AELVDAVLDV VRKEAESCDC LQGFQLTHSL GGGTGSGMGT LLISKIREEF PDRIMNTFSV VPSPKVSDTV VEPYNATLSV HQLVENTDET YCIDNEALYD ICFRTLKLTT PTYGDLNHLV SATMSGVTTC LRFPGQLNAD LRKLAVNMVP FPRLHFFMPG FAPLTSRGSQ QYRALTVPEL TQQMFDAKNM MAACDPRHGR YLTVAAVFRG RMSMKEVDEQ MLSVQSKNSS YFVEWIPNNV KTAVCDIPPR GLKMAATFIG NSTAIQELFK RISEQFTAMF RRKAFLHWYT GEGMDEMEFT EAESNMNDLV SEYQQYQDAT AEEGEFEEEA EEEVAVDLQS R
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
DisclaimerFor research use only

Alternative Names

TUBB4; TUBB5; Tubulin beta-4A chain; Tubulin 5 beta; Tubulin beta-4 chain

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human TUBB4A protein (orb392640)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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